There are many programs out there that claim to be able to remove spyware, but most of them are ineffective at best and spyware themselves at worse. Therefore, let me suggest my two favorite programs. I would suggest using them in conjunction with eachother, as what one program might miss the other one will pickup.
Spybot Search & Destroy is a nice little program which is 100% free. The author asks for a donation for using his product, but it is completely optional. Besides detects spyware and malware this program allows you to 'immunaize' your computer from most of the threats, as well as (optionally) running special protection with internet explorer.
Ad-Aware SE is the free version of Lavasoft's Ad-Aware product. It contains many features (though a few of the more advanced ones are disabled as an insentive foryou to buy the full version), and detects a broad range of threats.
Lastly, if you still use a browser like Internet Explorer I strongly suggest that you switch over to Firefox, as it is much much more secure and will stop many threats before they even reach your computer.
These programs, combiened, should keep your computer pretty much spyware/malware free. Though none of this is an alternative to good browsing habbits. Only download/install things from sites that look legitament. And never click on an email attachment from someone you don't know.

