If your plan is to use the old HD to format and install fresh OS, or use it as a backup/storage space the answer is yes (as mentioned above assuming the old HD and new motherboard are compatible).
However, if you are under the impression that the old HD with old OS will work with the new motherboard and new PC configuration chances are very slim. Since the Windows 2000, Microsoft's HCL made sure that you can't just swap any HD and make it work (thank a lot Bill

). The last OS to do this was Windows 98SE. And we all know what kind of junk Windows ME was... and I'm beginning to feel that Windows Vista may share the much "boos" as Windows ME.
All other *nix OS may recover from one set of hardware profile to another without giving you a headache.
FolkRockFan
I, too, picked up miniIDE/IDE/SATA to USB cable converter and it has made my PC repair gig much easier. God bless the geeks for thinking that one up

It's about time!
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